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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKN1A / WAF1 / p21 Antibody (aa133‑164) LS‑C344279
P21 antibody LS-C344279 is an unconjugated rabbit polyclonal antibody to p21 (CDKN1A / WAF1) from human and mouse. Validated for IF and WB.
Catalog
Size
Price
LS-C344279-400
400 µl
$305
Description
P21 antibody LS-C344279 is an unconjugated rabbit polyclonal antibody to p21 (CDKN1A / WAF1) from human and mouse. Validated for IF and WB.
Target
Human CDKN1A / WAF1 / p21
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:25)
  • Western blot (1:1000)
Epitope
aa133-164
Specificity
This CDKN1A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 133-164 amino acids from the C-terminal region of human CDKN1A.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About CDKN1A / WAF1 / p21
P38936 NM_078467 NP_000380.1

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Formalin-fixed, paraffin-embedded human colon cancer stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Western blot blot of extracts of various cell lines, using CDKN1A antibody.
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Immunohistochemistry Image
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p21 antibody WA-1 immunohistochemistry.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Formalin-fixed, paraffin-embedded bladder carcinoma stained with p21 antibody (HJ21).  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Images

Immunofluorescence

Fluorescent image of A549 cells stained with CDKN1A Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm
Fluorescent image of A549 cells stained with CDKN1A Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm

Western blot

Western blot of lysates from HeLa, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate (from left to right) with CDKN1A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Western blot of lysates from HeLa, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate (from left to right) with CDKN1A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Immunofluorescence

Fluorescent image of A549 cells stained with CDKN1A Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm
Fluorescent image of A549 cells stained with CDKN1A Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm

Western blot

Western blot of lysates from HeLa, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate (from left to right) with CDKN1A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Western blot of lysates from HeLa, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate (from left to right) with CDKN1A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Immunofluorescence

Fluorescent image of A549 cells stained with CDKN1A Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm
Fluorescent image of A549 cells stained with CDKN1A Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm

Western blot

Western blot of lysates from HeLa, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate (from left to right) with CDKN1A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Western blot of lysates from HeLa, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate (from left to right) with CDKN1A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Immunofluorescence

Fluorescent image of A549 cells stained with CDKN1A Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm
Fluorescent image of A549 cells stained with CDKN1A Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red). Cytoplasm

Western blot

Western blot of lysates from HeLa, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate (from left to right) with CDKN1A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Western blot of lysates from HeLa, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate (from left to right) with CDKN1A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Requested From: United States
Date Requested: 12/9/2018
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