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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDK6 Antibody (clone 8H4) IHC‑plus™ LS‑B5388
Note: This antibody replaces LS-C133112
CDK6 antibody LS-B5388 is an unconjugated mouse monoclonal antibody to CDK6 from human and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5388-50
50 µg (1 mg/ml)
Unavailable

Popular CDK6 Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation
Immunohistochemistry of paraffin-embedded human colon carcinoma tissue using CDK6 (aa11-15) antibody (left) or the same antibody preincubated with blocking peptide (right).
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence

Product Description

CDK6 antibody LS-B5388 is an unconjugated mouse monoclonal antibody to CDK6 from human and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CDK6
Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Q00534 NM_001259 NP_001250.1

CDK6 Antibody, CDKN6 Antibody, Cell division protein kinase 6 Antibody, Cyclin-dependent kinase 6 Antibody, Crk2 Antibody, PLSTIRE Antibody


Specifications

Target
Human CDK6
Host
Mouse
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [8H4]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDK6 antibody was raised against cDK6 (NP_001250, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Cdk6
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CDK6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5388 concentration 5 ug/ml.
Anti-CDK6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5388 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

CDK6 monoclonal antibody (M01), clone 8H4. Western blot of CDK6 expression in PC-12.
CDK6 monoclonal antibody (M01), clone 8H4. Western blot of CDK6 expression in PC-12.

Western blot

CDK6 monoclonal antibody (M01), clone 8H4 Western blot of CDK6 expression in Jurkat.
CDK6 monoclonal antibody (M01), clone 8H4 Western blot of CDK6 expression in Jurkat.

Western blot

Western blot of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.
Western blot of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.

Immunohistochemistry

Anti-CDK6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5388 concentration 5 ug/ml.
Anti-CDK6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5388 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

CDK6 monoclonal antibody (M01), clone 8H4. Western blot of CDK6 expression in PC-12.
CDK6 monoclonal antibody (M01), clone 8H4. Western blot of CDK6 expression in PC-12.

Western blot

CDK6 monoclonal antibody (M01), clone 8H4 Western blot of CDK6 expression in Jurkat.
CDK6 monoclonal antibody (M01), clone 8H4 Western blot of CDK6 expression in Jurkat.

Western blot

Western blot of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.
Western blot of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.

Immunohistochemistry

Anti-CDK6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5388 concentration 5 ug/ml.
Anti-CDK6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5388 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

CDK6 monoclonal antibody (M01), clone 8H4. Western blot of CDK6 expression in PC-12.
CDK6 monoclonal antibody (M01), clone 8H4. Western blot of CDK6 expression in PC-12.

Western blot

CDK6 monoclonal antibody (M01), clone 8H4 Western blot of CDK6 expression in Jurkat.
CDK6 monoclonal antibody (M01), clone 8H4 Western blot of CDK6 expression in Jurkat.

Western blot

Western blot of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.
Western blot of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.

Immunohistochemistry

Anti-CDK6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5388 concentration 5 ug/ml.
Anti-CDK6 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5388 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

CDK6 monoclonal antibody (M01), clone 8H4. Western blot of CDK6 expression in PC-12.
CDK6 monoclonal antibody (M01), clone 8H4. Western blot of CDK6 expression in PC-12.

Western blot

CDK6 monoclonal antibody (M01), clone 8H4 Western blot of CDK6 expression in Jurkat.
CDK6 monoclonal antibody (M01), clone 8H4 Western blot of CDK6 expression in Jurkat.

Western blot

Western blot of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.
Western blot of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.


Requested From: 
Date Requested: 6/20/2018

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