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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDK5R1 Antibody (C‑Terminus) LS‑C145910
CDK5R1 antibody LS-C145910 is an unconjugated rabbit polyclonal antibody to CDK5R1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C145910-50
50 µg
$365

Popular CDK5R1 Products

Immunofluorescent analysis of p35 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Bovine, Zebrafish
Applications: ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, ELISA
Western blot of tissue lysates using 2 ug/ml Rabbit Anti-p35 Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 35 KD. Observed Size: 35 KD.
Species:
Applications: Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Western blot of p35 (Q31) pAb in extracts from rat brain.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot

Product Description

CDK5R1 Antibody (C-Terminus) for WB/Western LS-C145910

Specifications

Target
Mouse CDK5R1
Synonyms
CDK5R1, CDK5R, CDK5P35, p35, p35nck5a, p23, p25, TPKII regulatory subunit, CDK5 activator 1, NCK5A, Neuronal CDK5 activator
Host
Rabbit
Reactivity
Mouse, Human, Rat, Bat, Bovine, Dog, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
CDK5R1 antibody was raised against synthetic peptide from C-Terminus of mouse Cdk5r1 (P61809, NP_034001). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Elephant, Panda, Horse, Guinea pig (100%); Monkey, Pig (92%).
Blocking Peptide
LS-E10520 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C145910. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Mouse CDK5R1 / p35
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CDK5R1
CDK5R1 (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. Q15078 NM_003885 NP_003876.1


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Images

Western blot

CDK5R1 / p35 antibody LS-C145910 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDK5R1 / p35 antibody LS-C145910 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CDK5R1 / p35 antibody LS-C145910 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDK5R1 / p35 antibody LS-C145910 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CDK5R1 / p35 antibody LS-C145910 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDK5R1 / p35 antibody LS-C145910 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CDK5R1 / p35 antibody LS-C145910 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDK5R1 / p35 antibody LS-C145910 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/16/2018

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