CDC27 | ANAPC3 | APC3 | CDC27 homolog | Cell division cycle 27 | D0S1430E | CDC27Hs | D17S978E | H-NUC | Nuc2 homolog | HNUC | NUC2
Human, Chimpanzee, Rat, Dog, Chicken
(tested or 100% immunogen sequence identity)
Chimpanzee, Rat, Dog, Chicken (at least 90% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of Human cdc27 amino acids 415-445 surrounding S426.
Reactivity occurs against human CDC27 pS426 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CDC27 is minimal by ELISA. The antibody does not cross-react with CDC27 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, chicken and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CDC27 protein from mouse may occur as sequence homology varies by one amino acid residue in this sequence (89% homology). Cross reactivity with CDC27 homologues from other sources has not been determined.
- Western blot (1:500 - 1:2500)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:25000)
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kD in size corresponding to CDC27 by western blotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS427 of CDC27.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.