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Catalog Number Size Price
LS-C18878-100 100 µg (1 mg/ml) $498 
CDC27 Antibody - Anti-cdc27 Antibody - Western Blot. Western blot of Affinity Purified anti-cdc27 antibody shows detection of a band ~90 kD corresponding to human cdc27 (arrowhead). Approximately 35 ug of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Human CDC27 Antibody (IF, WB) LS‑C18878

Polyclonal Rabbit anti‑Human CDC27 Antibody (IF, WB) LS‑C18878

Note: This antibody replaces LS-C78933
Antibody:
CDC27 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18878-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CDC27 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CDC27 antibody LS-C18878 is an unconjugated rabbit polyclonal antibody to human CDC27. Validated for ELISA, IF and WB.
Target
Human CDC27
Synonyms
CDC27 | ANAPC3 | APC3 | CDC27 homolog | Cell division cycle 27 | D0S1430E | CDC27Hs | D17S978E | H-NUC | Nuc2 homolog | HNUC | NUC2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 415-445 of Human cdc27.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, chicken, rat and dog based on 100% homology for the immunogen sequence. Expect cross reactivity with cdc27 from mouse as only a single amino acid residue change is found for the immunogen sequence. Cross reactivity with cdc27 homologues from other sources has not been determined.
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:40000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kD in size corresponding to Cdc27 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC27
P30260 NM_001256 NP_001247.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022