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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25C Antibody LS-C746941

Immunofluorescence analysis of U2OS cells using CDC25C antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CDC25C antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Immunofluorescence analysis of U2OS cells using CDC25C antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CDC25C antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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CDC25C Antibody LS-C746941

Antibody:
Rabbit Polyclonal to Human CDC25C
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C746941-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CDC25C
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CDC25C antibody LS-C746941 is an unconjugated rabbit polyclonal antibody to CDC25C from human and mouse. Validated for IF and WB.

Target
Human CDC25C
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Immunogen
CDC25C antibody was raised against recombinant fusion protein containing a sequence corresponding to amino acids 174-473 of human CDC25C (NP_001781.2). PKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLKKPIVPLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVKDMSP
Usage
The predicted MW is 45kDa/48kDa/50kDa/53kDa, while the observed MW by Western blot was 40kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC25C
P30307 NM_001790 NP_001781.2

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Reactivity: Human
Range: 0.313-20 ng/ml
Reactivity: Mouse
Range: 15.6-1000 pg/ml
Product
Species: Pig
Applications: Flow Cytometry

Requested From: United States
Date Requested: 6/18/2019
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