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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25C Antibody (FITC) LS‑C534859
CDC25C antibody LS-C534859 is an FITC-conjugated mouse monoclonal antibody to human CDC25C. Validated for ICC, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C534859-100
100 µl
$755

Popular CDC25C Products

Immunohistochemical analysis of CDC25C (pS216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunofluorescence analysis of U2OS cells.
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Anti-CDC25C antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemical analysis of CDC25C staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA, RNA interference

Product Description

CDC25C antibody LS-C534859 is an FITC-conjugated mouse monoclonal antibody to human CDC25C. Validated for ICC, IHC, IP and WB.

Specifications

Target
Human CDC25C
Synonyms
CDC25C, CDC25, CDC25C phosphatase, CDC25Hu3, Cell division cycle 25C, Phosphotyrosine phosphatase, PPP1R60, M-phase inducer phosphatase 3, Mitosis inducer CDC25
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
FITC. Also available Unconjugated or conjugated with AP, APC, Biotin, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • ICC
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDC25C antibody was raised against gST fusion-protein corresponding to residues 1-258 of human cdc25C. Epitope mapped to residues 222-246.
Specificity
Recognizes human cdc25C doublet at 56kD and 59kD, as well as proteins at 40kD and 35kD. Species cross-reactivity: Human.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.
About CDC25C
CDC25C, a CDC25 dual specificity protein phosphatase, has been shown to affect the regulation of cell cycle control, and specifically the G2/M progression, through interaction with cdc/cyclin kinases and proliferating cell nuclear antigen (PCNA). Unlike CDC25A and CDC25B, CDC25C does not seem to have oncogenic properties. CDC25C null mutant mice are viable, fertile, and do not display obvious abnomalities. P30307 NM_001790 NP_001781.2


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Requested From: United States
Date Requested: 11/14/2018

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