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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25C Antibody (clone 1F12) IHC-plus™ LS-B4149

Note: This antibody replaces LS-C109136
Anti-CDC25C antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot using anti-CDC25C monoclonal antibody against HeLa (1), K562 (2), PC-3 (3), HEK293 (4) and Raw264.7 (5) cell lysate.
Anti-CDC25C antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot using anti-CDC25C monoclonal antibody against HeLa (1), K562 (2), PC-3 (3), HEK293 (4) and Raw264.7 (5) cell lysate.
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CDC25C Antibody (clone 1F12) IHC-plus™ LS-B4149

Note: This antibody replaces LS-C109136
Antibody:
Mouse Monoclonal to Human CDC25C
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B4149-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human CDC25C
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CDC25C antibody LS-B4149 is an unconjugated mouse monoclonal antibody to human CDC25C. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human CDC25C
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1F12
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDC25C antibody was raised against purified recombinant fragment of human CDC25C expressed in E. Coli.\
Specificity
Human
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC25C
P30307 NM_001790 NP_001781.2

Validation

Anti-CDC25C antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-CDC25C antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CDC25C antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS.
Species: Human
Applications: Western blot
Reactivity: Mouse
Range: 0.312-20 ng/ml
Reactivity: Human, Mouse, Rat
Range: Positive/Negative

Requested From: United States
Date Requested: 6/18/2019
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