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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25C Antibody (APC) LS‑C254261
CDC25C antibody LS-C254261 is an APC-conjugated rabbit polyclonal antibody to human CDC25C. Validated for ELISA, Flow, IHC and WB.
Antibody
Catalog
Size
Price
LS-C254261-200
200 µl
$680

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Immunohistochemical analysis of CDC25C staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of CDC25C (pS216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

CDC25C antibody LS-C254261 is an APC-conjugated rabbit polyclonal antibody to human CDC25C. Validated for ELISA, Flow, IHC and WB.
About CDC25C
CDC25C, a CDC25 dual specificity protein phosphatase, has been shown to affect the regulation of cell cycle control, and specifically the G2/M progression, through interaction with cdc/cyclin kinases and proliferating cell nuclear antigen (PCNA). Unlike CDC25A and CDC25B, CDC25C does not seem to have oncogenic properties. CDC25C null mutant mice are viable, fertile, and do not display obvious abnomalities. P30307 NM_001790 NP_001781.2

CDC25C Antibody, CDC25 Antibody, CDC25C phosphatase Antibody, CDC25Hu3 Antibody, Cell division cycle 25C Antibody, Phosphotyrosine phosphatase Antibody, PPP1R60 Antibody, M-phase inducer phosphatase 3 Antibody, Mitosis inducer CDC25 Antibody


Specifications

Target
Human CDC25C
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Allophycocyanin. Also available Unconjugated or conjugated with AP, Biotin, FITC, HRP, PE.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • Flow Cytometry
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDC25C antibody was raised against recombinant human cdc25C.
Specificity
Human CDC25C
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, no preservatives added
Storage
Short term: store at 4°C. Long term: aliquot and store -20°C for up to 6 months. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 7/21/2018

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