CDC25C antibody LS-C5921 is an unconjugated mouse monoclonal antibody to human CDC25C. Validated for IHC, IP and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot
- (applications tested for the base form of this product only)
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CDC25C antibody was raised against gST fusion-protein corresponding to residues 1-258 of human cdc25C. Epitope mapped to residues 222-246.
Recognizes human cdc25C doublet at 56kD and 59kD, as well as proteins at 40kD and 35kD. Species cross-reactivity: Human.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. (1 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate cdc25C. Immunohistochemistry: Reported to immunostain cdc25C.
0.1 M Tris-glycine, pH 7.4, 0.105 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.