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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25B Antibody (Internal, clone EPR3459(2)) IHC‑plus™ LS‑B6243
Note: This antibody replaces LS-C138451
CDC25B antibody LS-B6243 is an unconjugated rabbit monoclonal antibody to human CDC25B. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6243-50
50 µl (0.487 mg/ml)
$425

Popular CDC25B Products

Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B931 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
CDC25B antibody LS-C29916 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Dog, Guinea pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence analysis of HUVEC cells, using CDC25B Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

CDC25B antibody LS-B6243 is an unconjugated rabbit monoclonal antibody to human CDC25B. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About CDC25B
CDC25B, a CDC25 dual specificity protein phosphatase, has been shown to affect cell cycle regulation by activating cyclin-dependent kinases Cdk1 and Cdk2 during G2 phase. CDC25B also has been shown to possess oncogenic properties and has been implicated in lung, breast, esophagus, stomach, ovarian, and other cancers. Recently CDC25B has been shown to be a steroid receptor coactivator and this function is independent of the protein phosphatase activity in vitro. P30305 NM_021872 NP_068658.1

CDC25B Antibody, CDC25B phosphatase Antibody, Cell division cycle 25B Antibody, M-phase inducer phosphatase 2 Antibody, CDC25HU2 Antibody


Specifications

Target
Human CDC25B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal [EPR3459(2)]
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot (1:10000 - 1:50000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDC25B antibody was raised against a synthetic peptide corresponding to residues in human Cdc25B.
Epitope
Internal
Specificity
Human CDC25B.
Presentation
Tris-Glycine-NaCl or PBS, pH 7.2-7.4, 40-50% Glycerol, 0.01% sodium azide, 0.05% BSA
Storage
Store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6243 dilution 1:50.
Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6243 dilution 1:50.

Western blot

Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.
Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.

Immunohistochemistry

Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6243 dilution 1:50.
Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6243 dilution 1:50.

Western blot

Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.
Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.

Immunohistochemistry

Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6243 dilution 1:50.
Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6243 dilution 1:50.

Western blot

Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.
Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.

Immunohistochemistry

Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6243 dilution 1:50.
Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6243 dilution 1:50.

Western blot

Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.
Western blot analysis on (A) K562, (B) U937, and (C) THP-1 cell lysates using anti-Cdc25B antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Requested From: United States
Date Requested: 9/24/2018

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