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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25B Antibody (aa56‑105) IHC‑plus™ LS‑B931
Note: This antibody replaces LS-C30342
CDC25B antibody LS-B931 is an unconjugated rabbit polyclonal antibody to CDC25B from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B931-50
50 µg
$485
Description
CDC25B antibody LS-B931 is an unconjugated rabbit polyclonal antibody to CDC25B from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CDC25B
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDC25B antibody was raised against synthetic peptide located between aa56-105 of human CDC25B (P30305, NP_068659). Percent identity by BLAST analysis: Human, Gorilla, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%); Monkey, Bat, Rabbit (92%); Mouse, Rat (85%).
Blocking Peptide
LS-E15204 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B931. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa56-105
Specificity
Human CDC25B
Usage
Immunohistochemistry: LS-B931 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B931 was determined to be 5 ug/ml.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CDC25B
P30305 NM_021872 NP_068658.1

Popular CDC25B Products

Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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CDC25B antibody AVARP03041_P050-NP_068659-CDC25B (cell division cycle 25 homolog B (S. pombe)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Immunofluorescence analysis of HUVEC cells, using CDC25B Antibody. The picture on the right is blocked with the synthesized peptide.
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Images

Immunohistochemistry

Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC25B antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/14/2018
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