Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (10 µg/ml)
- Western blot
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CDC25A antibody was raised against synthetic peptide from human CDC25A.
Amino acids surrounding Ser 75 of human CDC25A
Immunohistochemistry: LS-B1463 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1463 was determined to be 10 ug/ml.
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Short term: -20°C; Long term: -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.