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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25A Antibody LS‑C331324
CDC25A antibody LS-C331324 is an unconjugated rabbit polyclonal antibody to CDC25A from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C331324-50
50 µl
$235
LS-C331324-100
100 µl
$315
LS-C331324-200
200 µl
$425

Popular CDC25A Products

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1463 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25A (Phospho-Ser178) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry Image
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Immunoprecipitation Image
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation

Product Description

CDC25A antibody LS-C331324 is an unconjugated rabbit polyclonal antibody to CDC25A from human, mouse and rat. Validated for IHC and WB.
About CDC25A
CDC25A, a CDC25 dual specificity protein phosphatase, has been shown to activate the cyclin-dependent kinases at different points of the cell cycle. CDC25A has been shown to affect S phase entry and possibly the initiation of mitosis. CDC25A dephosphorylates cyclin dependent kinases, including Cdk2, Cdk4, as well as non cyclin proteins such as the homeodomain transcription factor cut, the proto-oncogene Raf-1. P30304 NM_001789 NP_001780.2

CDC25A Antibody, CDC25A2-CAG isoform Antibody, Cell division cycle 25A Antibody, CDC25A phosphatase Antibody, CDC25Hu1 Antibody, M-phase inducer phosphatase 1 Antibody, CDC25A2 Antibody


Specifications

Target
Human CDC25A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDC25A antibody was raised against recombinant fusion protein containing a sequence corresponding to amino acids 235-484 of human CDC25A (NP_963861.1). RPERSQEESPPGSTKRRKSMSGASPKESTNPEKAHETLHQSLSLASSPKGTIENILDNDPRDLIGDFSKGYLFHTVAGKHQDLKYISPEIMASVLNGKFANLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKKPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFMKCQSYCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL
Specificity
Human CDC25A
Usage
The predicted MW is 54kDa/59kDa, while the observed MW by Western blot was Refer to Figures.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) rat liver using CDC25A antibody at dilution of 1:100 (40x magnification).
Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) rat liver using CDC25A antibody at dilution of 1:100 (40x magnification).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) rat liver using CDC25A antibody at dilution of 1:100 (40x magnification).
Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) rat liver using CDC25A antibody at dilution of 1:100 (40x magnification).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) rat liver using CDC25A antibody at dilution of 1:100 (40x magnification).
Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) rat liver using CDC25A antibody at dilution of 1:100 (40x magnification).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibodyat dilution of 1:100 (40x lens).

Immunohistochemistry

Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) rat liver using CDC25A antibody at dilution of 1:100 (40x magnification).
Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) rat liver using CDC25A antibody at dilution of 1:100 (40x magnification).

Requested From: United States
Date Requested: 9/23/2018

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