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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25A Antibody (aa271‑300) IHC‑plus™ LS‑B3457
Note: This antibody replaces LS-C99977
CDC25A antibody LS-B3457 is an unconjugated rabbit polyclonal antibody to human CDC25A. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3457-200
200 µl
$425
Description
CDC25A antibody LS-B3457 is an unconjugated rabbit polyclonal antibody to human CDC25A. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CDC25A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8167 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3457. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa271-300
Specificity
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human Cdc25A.
Usage
Immunohistochemistry: LS-B3457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3457 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About CDC25A
P30304 NM_001789 NP_001780.2

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Images

Immunohistochemistry

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.

Immunohistochemistry

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.

Immunohistochemistry

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.

Immunohistochemistry

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 12/11/2018
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