CDC25A antibody LS-B3457 is an unconjugated rabbit polyclonal antibody to human CDC25A. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human Cdc25A.
Immunohistochemistry: LS-B3457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3457 was determined to be 10 ug/ml.
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.