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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25A Antibody (aa271‑300) IHC‑plus™ LS‑B3457
Note: This antibody replaces LS-C99977
CDC25A antibody LS-B3457 is an unconjugated rabbit polyclonal antibody to human CDC25A. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3457-200
200 µl
$425

Popular CDC25A Products

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1463 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25A (Phospho-Ser178) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry Image
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Immunoprecipitation Image
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation

Product Description

CDC25A antibody LS-B3457 is an unconjugated rabbit polyclonal antibody to human CDC25A. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CDC25A
CDC25A, a CDC25 dual specificity protein phosphatase, has been shown to activate the cyclin-dependent kinases at different points of the cell cycle. CDC25A has been shown to affect S phase entry and possibly the initiation of mitosis. CDC25A dephosphorylates cyclin dependent kinases, including Cdk2, Cdk4, as well as non cyclin proteins such as the homeodomain transcription factor cut, the proto-oncogene Raf-1. P30304 NM_001789 NP_001780.2

CDC25A Antibody, CDC25A2-CAG isoform Antibody, Cell division cycle 25A Antibody, CDC25A phosphatase Antibody, CDC25Hu1 Antibody, M-phase inducer phosphatase 1 Antibody, CDC25A2 Antibody


Specifications

Target
Human CDC25A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8167 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3457. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa271-300
Specificity
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human Cdc25A.
Usage
Immunohistochemistry: LS-B3457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3457 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3457 concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3457 concentration 10 ug/ml.

Western blot

Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.

Immunohistochemistry

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3457 concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3457 concentration 10 ug/ml.

Western blot

Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.

Immunohistochemistry

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3457 concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3457 concentration 10 ug/ml.

Western blot

Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.

Immunohistochemistry

Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3457 concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3457 concentration 10 ug/ml.

Western blot

Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 9/25/2018

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