(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000)
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- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3457. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human Cdc25A.
Immunohistochemistry: LS-B3457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3457 was determined to be 10 ug/ml.
PBS, 0.09% sodium azide
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.