Mouse CD8A / CD8 Alpha

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

53-6.7

Unconjugated

Protein G purified

Unmodified

Mouse thymus or spleen

Detects mouse CD8a, the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. Has also been used for depletion of CD8a+ cells.

• IHC - Frozen
• Immunofluorescence
• Immunoprecipitation
• Flow Cytometry

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Immunohistochemistry: LS-C45021 has been reported as working well on Zinc-fixed paraffin-embedded sections. However, inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01732 NCBI: NM_001768 NP_001759.3