Human CD74 / CLIP

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Bu45

FITC

Protein G purified

Unmodified

B lymphoblastoid cell line HFB1

C-Terminus

Recognizes human CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Recognizes 33, 35 and 41 kD isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus. *Membrane permeabilization may enhance CD74 staining on monocytes but is not required when staining lymphocytes. XXX recommend the use of Leucoperm (product code BUF09) for this purpose.

• Flow Cytometry (1:1 - 1:5)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.09% Sodium Azide, 1% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04233 NCBI: NM_004355 NP_004346.1