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Catalog Number	Size	Price
LS-C662590-10	10 µg	$318
LS-C662590-100	100 µg	$470

CD7 Antibody - Immunohistochemistry - Anti-CD7 Picoband antibody

CD7 Antibody - Western blot analysis of CD7 using anti-CD7 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CD7 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD7 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD7 at approximately 30-40KD. The expected band size for CD7 is at 18KD.

CD7 Antibody - Flow Cytometry analysis of PBMC cells using anti-CD7 antibody. Overlay histogram showing PBMC cells stained with anti-CD7 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD7 Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human CD7 Antibody (IHC, WB) LS‑C662590

Name: Anti-CD7 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662590-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CD7 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, ICC, WB, Flo, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662590-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD7 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, ICC, WB, Flo, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CD7 antibody LS-C662590 is an unconjugated rabbit polyclonal antibody to human CD7. Validated for ELISA, Flow, ICC, IHC and WB.

Target

Human CD7

Synonyms

CD7 | CD7 antigen | CD7 antigen (p41) | CD7 molecule | LEU-9 | T-cell leukemia antigen | T-cell antigen CD7 | T-cell surface antigen Leu-9 | TP41 | gp40 | p41 protein | Tp40

See All CD7 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Modifications

Unmodified

Immunogen

E. coli-derived human CD7 recombinant protein (Position: A26-D172).

Applications

IHC
IHC - Paraffin
ICC
Western blot
Flow Cytometry
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD7

Uniprot: P09564 NCBI: NM_006137 NP_006128.1