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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD69 Antibody (clone H1.2F3, Biotin) LS‑C106250
CD69 antibody LS-C106250 is a biotin-conjugated hamster monoclonal antibody to mouse CD69. Validated for Flow.
Catalog
Size
Price
LS-C106250-50
50 µg
$215
LS-C106250-100
100 µg
$295
LS-C106250-500
500 µg
$355
Description
CD69 antibody LS-C106250 is a biotin-conjugated hamster monoclonal antibody to mouse CD69. Validated for Flow.
Target
Mouse CD69
Host
Hamster
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [H1.2F3]
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, PE, APC, PE, Cy7, PE, Cy5.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Immunogen
CD69 antibody was raised against mouse CD69
Usage
The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
About CD69
Q07108 NM_001781 NP_001772.1

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Images

Flow Cytometry

Staining of ConA-activated BALB/c splenocytes with 0.25 ug of Biotin Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Biotin anti-mouse CD69 (H1.2F3) (colored histogram) followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of ConA-activated BALB/c splenocytes with 0.25 ug of Biotin Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Biotin anti-mouse CD69 (H1.2F3) (colored histogram) followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of ConA-activated BALB/c splenocytes with 0.25 ug of Biotin Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Biotin anti-mouse CD69 (H1.2F3) (colored histogram) followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of ConA-activated BALB/c splenocytes with 0.25 ug of Biotin Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Biotin anti-mouse CD69 (H1.2F3) (colored histogram) followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of ConA-activated BALB/c splenocytes with 0.25 ug of Biotin Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Biotin anti-mouse CD69 (H1.2F3) (colored histogram) followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of ConA-activated BALB/c splenocytes with 0.25 ug of Biotin Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Biotin anti-mouse CD69 (H1.2F3) (colored histogram) followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of ConA-activated BALB/c splenocytes with 0.25 ug of Biotin Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Biotin anti-mouse CD69 (H1.2F3) (colored histogram) followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of ConA-activated BALB/c splenocytes with 0.25 ug of Biotin Armenian Hamster IgG isotype control (open histogram) or 0.25 ug of Biotin anti-mouse CD69 (H1.2F3) (colored histogram) followed by SAv-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/13/2018
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