CD59 antibody LS-C5454 is an unconjugated mouse monoclonal antibody to human CD59. Validated for ELISA, Flow and IF.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Immunofluorescence (1:25)
- Flow Cytometry (1:25)
- (applications tested for the base form of this product only)
CD59 antibody was raised against human erythrocytes.
Binds to a 17-25kD glycoprotein which is distributed on human cells and has a glycosyl-phosphatidylinositol (GPI) linkage to the plasma membrane. Cell reactivity: Recognizes all leucocytes, platelets, fibroblasts, erythrocytes and most cell lines. It is negative on U937 and Daudi cell lines. It is reduced on affected cells from patients with PNH with a distribution reflecting the clonal nature of the disease.
Suitable for use in Flow Cytometry, Indirect Immunofluorescence, and ELISA. Flow Cytometry: 1:25. Indirect Immunofluorescence: 1:25. Dilute with isotonic buffer. Use 50 ul of the appropriate dilution per 1x1E6 cells in 100 ul buffer.
0.2 M PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
Short term: -20°C; Long term: -20°C.
For research use only.