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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD59 Antibody (clone p282(H19)) IHC‑plus™ LS‑B1960
Note: This antibody replaces LS-C41091
CD59 antibody LS-B1960 is an unconjugated mouse monoclonal antibody to CD59 from human, baboon and monkey. Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1960-50
50 µg (0.5 mg/ml)
$425

Popular CD59 Products

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Species: Human
Applications: Flow Cytometry
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Product Description

CD59 antibody LS-B1960 is an unconjugated mouse monoclonal antibody to CD59 from human, baboon and monkey. Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
About CD59
CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. P13987 NM_000611 NP_000602.1

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Specifications

Target
Human CD59
Host
Mouse
Reactivity
Human, Baboon, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [p282(H19)]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Flow Cytometry (2 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1960 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1960 was determined to be 10 ug/ml.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. Sourced in TCS.
Storage
Store at 4°C. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.
Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.

Flow Cytometry

Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC
Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC

Immunohistochemistry

Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.
Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.

Flow Cytometry

Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC
Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC

Immunohistochemistry

Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.
Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.

Flow Cytometry

Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC
Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC

Immunohistochemistry

Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.
Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.

Flow Cytometry

Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC
Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC

Requested From: United States
Date Requested: 8/15/2018

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