CD59 antibody LS-B2106 is an unconjugated mouse monoclonal antibody to human CD59. Validated for ELISA, Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Immunohistochemistry: LS-B2106 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2106 was determined to be 5 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Tonsil.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.