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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD59 Antibody (clone MEM‑43/5) IHC‑plus™ LS‑B1853
Note: This antibody replaces LS-C45838
CD59 antibody LS-B1853 is an unconjugated mouse monoclonal antibody to CD59 from human and mouse. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1853-50
50 µg (1 mg/ml)
$425

Popular CD59 Products

Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1960 concentration 10 ug/ml.
Species: Human, Baboon, Monkey
Applications: IHC, IHC - Paraffin, Flow Cytometry
Antibody
Species: Human
Applications: Flow Cytometry
Antibody
Species: Rat
Applications: IHC, IHC - Frozen, Western blot, Flow Cytometry, Functional Assay
Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1619 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry

Product Description

CD59 antibody LS-B1853 is an unconjugated mouse monoclonal antibody to CD59 from human and mouse. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human CD59
Synonyms
CD59, 16.3A5, 1F5, 1F5 antigen, CD59 glycoprotein, EL32, EJ16, G344, HRF-20, MAC-IP, MACIF, Ly-6-like protein, MEM43, MIRL, MEM43 antigen, MIN2, MIN3, MSK21, p18-20, Protectin, MAC-inhibitory protein, MIC11, T cell-activating protein, CD59 antigen, EJ30, HRF20, Human leukocyte antigen MIC11, Lymphocytic antigen CD59/MEM43, MIN1
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [MEM-43/5]
Conjugations
Unconjugated
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry (1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD59 antibody was raised against thymocytes and T lymphocytes
Specificity
The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.
Usage
Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only.
About CD59
CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. P13987 NM_000611 NP_000602.1


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Images

Immunohistochemistry

Anti-CD59 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1853 concentration 5 ug/ml.
Anti-CD59 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1853 concentration 5 ug/ml.

Western blot

Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5).  Lane 1: original cell lysate  Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)
Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)

Immunohistochemistry

Anti-CD59 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1853 concentration 5 ug/ml.
Anti-CD59 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1853 concentration 5 ug/ml.

Western blot

Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5).  Lane 1: original cell lysate  Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)
Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)

Immunohistochemistry

Anti-CD59 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1853 concentration 5 ug/ml.
Anti-CD59 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1853 concentration 5 ug/ml.

Western blot

Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5).  Lane 1: original cell lysate  Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)
Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)

Immunohistochemistry

Anti-CD59 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1853 concentration 5 ug/ml.
Anti-CD59 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1853 concentration 5 ug/ml.

Western blot

Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5).  Lane 1: original cell lysate  Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)
Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43)

Requested From: United States
Date Requested: 10/20/2018

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