Human CD59

Rabbit

Human (tested or 100% immunogen sequence identity)

Baboon, Horse

IgG Recombinant Monoclonal

MACIF/2867R

Unconjugated

Purified

Unmodified. Also available Azide-free, Carrier-free.

Recombinant full-length human CD59 protein. Antigen Molecular Weight: 20 kDa

Reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI; Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.

• IHC - Paraffin (1 - 2 µg/ml)

Flow Cytometry (0.5-1 ug/million cells) - Immunofluorescence (0.5-1 ug/ml) - Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13987 NCBI: NM_000611 NP_000602.1