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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD55 Antibody (clone F4‑29D9, Azide‑free) LS‑C390053
CD55 antibody LS-C390053 is an unconjugated mouse monoclonal antibody to human CD55. Validated for Flow and IF.
Antibody
Catalog
Size
Price
LS-C390053-100
100 µg (1 mg/ml)
$415

Popular CD55 Products

Antibody
Species: Mouse
Applications: Western blot, Flow Cytometry, ELISA
Anti-CD55 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1851 concentration 10 ug/ml.
Species: Human, Primate
Applications: IHC, IHC - Paraffin, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Western blot
Anti-CD55 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4045 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Flow Cytometry

Product Description

CD55 antibody LS-C390053 is an unconjugated mouse monoclonal antibody to human CD55. Validated for Flow and IF.
About CD55
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. P08174 NM_000574 NP_000565.1

CD55 Antibody, CD55 antigen Antibody, Decay-accelerating factor Antibody, TC Antibody, CR Antibody, CROM Antibody, DAF Antibody


Specifications

Target
Human CD55
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [F4-29D9]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified.
Applications
  • Immunofluorescence (1 - 2 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Immunogen
CD55 antibody was raised against human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the CD55 antibody.
Specificity
Human CD55
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). This mAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH). Optimal dilution of the CD55 antibody should be determined by the researcher.
Presentation
Sterile Solution PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/16/2018

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