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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD53 Antibody LS‑C5321
CD53 antibody LS-C5321 is an unconjugated rat monoclonal antibody to CD53 from mouse and rat. Validated for Flow.
Antibody
Catalog
Size
Price
LS-C5321-2000
2000 µl
$530

Popular CD53 Products

Antibody
Species: Human
Applications: Flow Cytometry
Anti-CD53 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunofluorescence, Flow Cytometry
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry

Product Description

CD53 antibody LS-C5321 is an unconjugated rat monoclonal antibody to CD53 from mouse and rat. Validated for Flow.

Specifications

Target
Rat CD53
Synonyms
CD53, CD53 antigen, CD53 molecule, CD53 tetraspan antigen, Cell surface antigen, Leukocyte surface antigen CD53, Transmembrane glycoprotein, Tetraspanin-25, Tspan-25, CD53 glycoprotein, Cell surface glycoprotein CD53, MOX44, TSPAN25
Host
Rat
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgM Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • Flow Cytometry
Immunogen
CD53 antibody was raised against rAW264 cell line.
Specificity
Recognizes mouse CD53 cell surface antigen Recognizes an extracellular epitope and its binding is dependent upon the threonine residue at position 154.
Usage
Suitable for use in Flow Cytometry. Flow Cytometry: Neat -1:5. Use 10 ul of the suggested working dilution to label 1E6 cells in 100ul.
Presentation
0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About CD53
CD53 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. P19397 NM_000560 NP_000551.1


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Requested From: United States
Date Requested: 11/14/2018

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