CD53 antibody LS-C5319 is an unconjugated mouse monoclonal antibody to rat CD53. Validated for Flow, IHC and IP.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin
- IHC - Frozen
- Flow Cytometry (1:100 - 1:200)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CD53 antibody was raised against rat T cell blasts.
Recognizes the rat CD53 cell surface antigen, a 43kD glycoprotein, which is a member of the transmembrane 4 super family. Clone OX-44 recognizes a conformational epitope that is dependent on disulphide bonding.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1E6 cells in 100ul. Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Applications: Flow Cytometry: 1:100-1:200. Immunohistochemistry: Frozen, Paraffin. Immunoprecipitation.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.