(tested or 100% immunogen sequence identity)
- IHC - Frozen
- Western blot
- Flow Cytometry
- Functional Assay
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CD53 antibody was raised against leukocytes of pacient suffering from a LGL-type leukemia.
The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 15 mM Sodium Azide
Store in the dark at 2°C to 8°C. Do not freeze. Protect from light.
For research use only.