CD53 antibody LS-C45705 is an unconjugated mouse monoclonal antibody to human CD53. Validated for Flow, Func, IHC, IP and WB.
(tested or 100% immunogen sequence identity)
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
- IHC - Frozen
- Western blot
- Flow Cytometry
- Functional Assay
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CD53 antibody was raised against leukocytes of pacient suffering from a LGL-type leukemia.
The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Flow Cytometry: Recommended dilution - 4 ug/ml. Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors. Functional Application: The antibody MEM-53 induces activation of monocytes and B lymphocytes. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.4. No preservative added (azide free)
Store at 2°C to 8°C. Do not freeze.
For research use only.