(tested or 100% immunogen sequence identity)
CD53 antibody was raised against human Sezary cells.
Flow Cytometry: 5x10e5 cultured human Jurkat cells per tube were incubated 45 min on ice with 80 ul of anti-CD53 antibody at 10 ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.37 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.
For research use only.