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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD53 Antibody (clone 161‑2, Azide‑free) LS‑C391287
CD53 antibody LS-C391287 is an unconjugated mouse monoclonal antibody to human CD53. Validated for Flow and IF.
Catalog
Size
Price
LS-C391287-100
100 µg (1 mg/ml)
$415
Description
CD53 antibody LS-C391287 is an unconjugated mouse monoclonal antibody to human CD53. Validated for Flow and IF.
Target
Human CD53
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Baboon, Horse
Clonality
IgG2a,k Monoclonal [161-2]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified.
Applications
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Immunogen
CD53 antibody was raised against stimulated human leukocytes were used as the immunogen for the CD53 antibody.
Specificity
Human CD53.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI. Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This mAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1). Optimal dilution of the CD53 antibody should be determined by the researcher.
Presentation
Sterile Solution PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
About CD53
P19397 NM_000560 NP_000551.1

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Requested From: United States
Date Requested: 11/19/2018
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