Target
Dog CD5
Synonyms
CD5 | CD5 antigen | CD5 antigen (p56-62) | CD5 molecule | Lymphocyte antigen T1/Leu-1 | LEU1 | T1
Reactivity
Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
R. Phycoerythrin.
Also available conjugated with
FITC,
APC.
Purification
Protein G purified
Immunogen
Concanavalin A activated canine peripheral blood cells.
Specificity
Recognizes canine CD5, a 67 kD cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels. Was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop. In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression. Serves as a useful marker for the discrimination of canine leukemias of differing origins.
Applications
- Flow Cytometry
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee