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Catalog Number Size Price
LS-C796669-100 100 µg $508 
CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse thymus tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:300).
CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120 oC for 3 min. (1:300).
CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:300).
CD5 Antibody - Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD5 clone UMAB9 mouse monoclonal antibody at 1:200 dilution of 0.1mg/mL detection with Polink2 Broad HRP DAB requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >75 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.
CD5 Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
CD5 Antibody - Immunofluorescent staining of Jurkat cells using CD5 mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
CD5 Antibody - Western blot analysis of extracts. (35ug) from 2 cell lines lysates by using anti-CD5 monoclonal antibody. (1:500)
CD5 Antibody - Western Blot analysis of 10 different human tissue lysates. (10ug) by using anti-CD5 monoclonal antibody. (clone UMAB9, 1:500)
CD5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CD5. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. (1:500)
CD5 Antibody - Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody. (Red), compared to a nonspecific negative control antibody. (Blue).
CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse thymus tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:300).
CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120 oC for 3 min. (1:300).
CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:300).
CD5 Antibody - Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD5 clone UMAB9 mouse monoclonal antibody at 1:200 dilution of 0.1mg/mL detection with Polink2 Broad HRP DAB requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >75 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.
CD5 Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
CD5 Antibody - Immunofluorescent staining of Jurkat cells using CD5 mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
CD5 Antibody - Western blot analysis of extracts. (35ug) from 2 cell lines lysates by using anti-CD5 monoclonal antibody. (1:500)
CD5 Antibody - Western Blot analysis of 10 different human tissue lysates. (10ug) by using anti-CD5 monoclonal antibody. (clone UMAB9, 1:500)
CD5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CD5. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. (1:500)
CD5 Antibody - Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody. (Red), compared to a nonspecific negative control antibody. (Blue).
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Monoclonal Mouse anti‑Human CD5 Antibody (clone UMAB9, Carrier‑free, IHC, IF, WB) LS‑C796669

Monoclonal Mouse anti‑Human CD5 Antibody (clone UMAB9, Carrier‑free, IHC, IF, WB) LS‑C796669

Antibody:
CD5 Mouse anti-Human Monoclonal (Carrier-free) (UMAB9) Antibody
Application:
IHC, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$508
LS-C796669-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD5 Mouse anti-Human Monoclonal (Carrier-free) (UMAB9) Antibody
Application:
IHC, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
CD5 antibody LS-C796669 is an unconjugated mouse monoclonal antibody to human CD5. Validated for Flow, IF, IHC and WB.
Target
Human CD5
Synonyms
CD5 | CD5 antigen | CD5 antigen (p56-62) | CD5 molecule | Lymphocyte antigen T1/Leu-1 | LEU1 | T1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
UMAB9
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:500)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with distilled water to 1 mg/ml. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD5
P06127 NM_014207 NP_055022.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/1/2022