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Catalog Number Size Price
LS-C392474-20 20 µg (0.2 mg/ml) $354 
LS-C392474-100 100 µg (0.2 mg/ml) $550 
CD5 Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD5 antibody (SPM546).  This image was taken for the unmodified form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human CD5 Antibody (clone SPM546, IHC, IF) LS‑C392474

Monoclonal Mouse anti‑Human CD5 Antibody (clone SPM546, IHC, IF) LS‑C392474

Antibody:
CD5 Mouse anti-Human Monoclonal (SPM546) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$354
LS-C392474-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD5 Mouse anti-Human Monoclonal (SPM546) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD5 antibody LS-C392474 is an unconjugated mouse monoclonal antibody to human CD5. Validated for Flow, IF and IHC.
Target
Human CD5
Synonyms
CD5 | CD5 antigen | CD5 antigen (p56-62) | CD5 molecule | Lymphocyte antigen T1/Leu-1 | LEU1 | T1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
SPM546
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free, BSA-free.
Immunogen
Human CD5 recombinant protein.
Specificity
Human CD5
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Recognizes a 67kDa transmembrane protein which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes. The optimal dilution of the anti-CD5 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD5
P06127 NM_014207 NP_055022.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/1/2022