Human CD45RO

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

UCHL-1 + T200/797

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (clone UCHL-1) and recombinant human CD45RO protein (clone T200/797) were used as the immunogens for this CD45RO antibody cocktail.

Human CD45RO

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Flow Cytometry

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody cocktail reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. Due to variation in protocol and secondary antibody used, the CD45RO antibody cocktail may need to be titered for optimal performance. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.