Mouse CD3E

Hamster

Mouse (tested or 100% immunogen sequence identity)

IgG Monoclonal

145-2C11

Unconjugated

Protein G purified

Unmodified

H-2K b - specific mouse cytotoxic T lymphocyte clone BM10-37.

Detects CD3 epsilon (CD3e), a 20kD transmembrane protein also known as CD3 or T3. CD3e is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell. CD3e is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.

• IHC - Frozen
• Immunofluorescence
• Western blot
• Immunoprecipitation
• Flow Cytometry

Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_000733 NP_000724.1