(tested or 100% immunogen sequence identity)
CD37 antibody was raised against iPO-24 was generated after immunization of BALB/c mice with spleen cells of a patient with hairy cell leukemia. Mice splenocytes were fused with the P3-X63-Ag8.653 mouse myeloma cell line.
The antigen CD37 defined by IPO-24 is B lineage restricted and is strongly expressed throughout B cell ontogeny from pre-B cells until the plasmoblasts. CD37 is an extensively glycosylated molecule and usually two and sometimes three N-linked carbohydrate chain are attached to the protein. IPO24 reacts with human B cell lines Daudi, Raji, Namalva, Ramos and CABIL, whereas T cell lines CEM, Molt-4, Jurkat, MT-4, H9 and non-lymphoid cell lines MeWo and A-431 are not labeled. IPO-24 stains B cell follicles and the most strongly labeled elements are mantle zone lymphoid cells. Neutrophils, macrophages, thymocytes and dendritic reticulum cells are negative.