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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD30L / CD153 Antibody (clone RM153, PE) LS‑C105988
CD153 antibody LS-C105988 is a PE-conjugated rat monoclonal antibody to mouse CD153 (CD30L). Validated for Flow.
Catalog
Size
Price
LS-C105988-50
50 µg
$225
LS-C105988-100
100 µg
$305
LS-C105988-200
200 µg
$355
Description
CD153 antibody LS-C105988 is a PE-conjugated rat monoclonal antibody to mouse CD153 (CD30L). Validated for Flow.
Target
Mouse CD30L / CD153
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [RM153]
Conjugations
Phycoerythrin. Also available Unconjugated or conjugated with Biotin.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Immunogen
CD30L / CD153 antibody was raised against mouse TNFSF8
Usage
The RM153 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About CD30L / CD153
P32971 NM_001244 NP_001235.1

Popular CD30L / CD153 Products

Western blot of recombinant CD30L / CD153.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, Western blot
Western Blot analysis of TNFSF8 expression in transfected 293T cell line by TNFSF8 monoclonal antibody (M01A), clone 2E11.Lane 1: TNFSF8 transfected lysate (Predicted MW: 26 KDa).Lane 2: Non-transfected lysate.
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Applications: Western blot, ELISA
Western blot of recombinant CD30L / CD153.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Western blot analysis of CD153 expression in Raw264.7 (A); rat kidney (B) whole cell lysates.
Species: Human, Mouse, Rat
Applications: Western blot

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Images

Flow Cytometry

Staining of 2-day ConA activated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of 2-day ConA activated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of non-stimulated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of non-stimulated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of 2-day ConA activated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of 2-day ConA activated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of non-stimulated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of non-stimulated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of 2-day ConA activated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of 2-day ConA activated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of non-stimulated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of non-stimulated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of 2-day ConA activated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of 2-day ConA activated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of non-stimulated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of non-stimulated BALB/c splenocytes with 0.06 ug PE rat IgG2b isotype control (open histogram) or 0.06 ug PE anti-mouse CD153 (RM153) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/13/2018
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