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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD300A Antibody (clone MEM‑260) LS‑C46165
CD300A antibody LS-C46165 is an unconjugated mouse monoclonal antibody to human CD300A. Validated for Flow and WB.
Catalog
Size
Price
LS-C46165-100
100 µg (1 mg/ml)
$265
Description
CD300A antibody LS-C46165 is an unconjugated mouse monoclonal antibody to human CD300A. Validated for Flow and WB.
Target
Human CD300A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [MEM-260]
Conjugations
Unconjugated. Also available conjugated with PE.
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Applications
  • Western blot
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Immunogen
CD300A antibody was raised against hPB human acute lymphoid leukemia cell line.
Specificity
The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
Usage
Flow Cytometry: Recommended dilution - 2 ug/ml. Western Blotting: Positive material: HPB cell line. Negative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylma
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only.
About CD300A
Q9UGN4 NM_007261 CAB52291.1

Popular CD300A Products

Human peripheral blood monocytes stained with Mouse anti-Human CD300a:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Human peripheral blood monocytes stained with Mouse anti-Human CD300a followed by Rabbit F(ab')2 anti-Mouse IgG:FITC
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Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.
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Human peripheral blood monocytes stained with Mouse anti-Human CD300a:RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Images

Flow Cytometry

Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.
Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.

Flow Cytometry

Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.
Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.

Flow Cytometry

Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.
Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.

Flow Cytometry

Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.
Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.

Requested From: United States
Date Requested: 12/18/2018
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