CD300A antibody LS-C46165 is an unconjugated mouse monoclonal antibody to human CD300A. Validated for Flow and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with PE
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
- Western blot
- Flow Cytometry
- (applications tested for the base form of this product only)
CD300A antibody was raised against hPB human acute lymphoid leukemia cell line.
The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
Flow Cytometry: Recommended dilution - 2 ug/ml. Western Blotting: Positive material: HPB cell line. Negative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylma
PBS, pH 7.4, 15 mM Sodium Azide
Store at 2°C to 8°C. Do not freeze.
For research use only.