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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD30 Antibody (aa19‑379, clone 3B5) LS‑C336396
CD30 antibody LS-C336396 is an unconjugated mouse monoclonal antibody to human CD30. Validated for IHC and WB.
Catalog
Size
Price
LS-C336396-100
100 µl (1 mg/ml)
$335

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Product Description

CD30 Antibody (aa19-379, clone 3B5) for IHC, WB/Western LS-C336396

Specifications

Target
Human CD30
Synonyms
TNFRSF8, CD30L receptor, CD30, Cytokine receptor CD30, D1S166E, Ki-1, Ki-1 antigen, CD30 antigen
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [3B5]
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD30 antibody was raised against human recombinant protein fragment corresponding to amino acids 19-379 of human TNFRSF8 (NP_001234) produced in E. coli.
Epitope
aa19-379
Specificity
Human CD30
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CD30
CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. P28908 NM_001243 NP_001234.3


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Images

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8.

Requested From: United States
Date Requested: 10/19/2018

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