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Antibodies > CD274 / B7-H1 / PD-L1 > LS-C793048
 
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Catalog Number Size Price
LS-C793048-100 100 µg $511 
CD274 / B7-H1 / PD-L1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CD274. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274. (1:500)
CD274 / B7-H1 / PD-L1 Antibody - Detection of PDL1 neutralizing antibody using MACS column. GFP+/PDL1+ 293T cells. (co-transfected with PDL1 and GFP plasmid PS10010) were incubated with either PDL1 antibody, non-specific antibody(green),isotype control(blue) or PBS(black) and then mixed with PD1+ 293T cells linked with magnetic-beads. The mixed cells were pulled down using MACS column. (Miltenyi Biotec) and analysed by Flow Cytometry. GFP+/PDL1+ cells would not be collected if PD1/PDL1 interaction is neutralized by the tested antibody. (1:50)
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometric analysis of living HCC78 cells, using anti-CD274 antibody Red), compared to an isotype control. (green), and a PBS control. (blue). (1:100)
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometric analysis of the stable expression of CD274 plasmid in living 293T cells using anti-PDL1 antibody red) , compared to an isotype control. (green), and a PBS control. (blue). (1:100)
CD274 / B7-H1 / PD-L1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CD274. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274. (1:500)
CD274 / B7-H1 / PD-L1 Antibody - Detection of PDL1 neutralizing antibody using MACS column. GFP+/PDL1+ 293T cells. (co-transfected with PDL1 and GFP plasmid PS10010) were incubated with either PDL1 antibody, non-specific antibody(green),isotype control(blue) or PBS(black) and then mixed with PD1+ 293T cells linked with magnetic-beads. The mixed cells were pulled down using MACS column. (Miltenyi Biotec) and analysed by Flow Cytometry. GFP+/PDL1+ cells would not be collected if PD1/PDL1 interaction is neutralized by the tested antibody. (1:50)
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometric analysis of living HCC78 cells, using anti-CD274 antibody Red), compared to an isotype control. (green), and a PBS control. (blue). (1:100)
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometric analysis of the stable expression of CD274 plasmid in living 293T cells using anti-PDL1 antibody red) , compared to an isotype control. (green), and a PBS control. (blue). (1:100)
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Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone OTI11G4, Carrier‑free, WB) LS‑C793048

Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone OTI11G4, Carrier‑free, WB) LS‑C793048

Submit Review LSBio Guarantee
Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (Carrier-free) (OTI11G4) Antibody
Application:
WB, Flo
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Unmodified
Price
Catalog Number
$511
LS-C793048-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (Carrier-free) (OTI11G4) Antibody
Application:
WB, Flo
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Unmodified

Specifications

Description
PD-L1 antibody LS-C793048 is an unconjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for Flow and WB.
Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
OTI11G4
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human CD274 (NP_054862) produced in HEK293T cell.
Applications
  • Western blot (1:500)
  • Flow Cytometry (1:50 - 1:100)
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Featured Products

CD274 / B7-H1 / PD-L1 Antibody
CD274 / B7‑H1 / PD‑L1 Antibody (clone 27A2) LS‑C179481
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD274 / B7‑H1 / PD‑L1 Antibody (clone ANC6H1, Biotin) LS‑C134637
Species: Human
Applications: Flow Cytometry, ELISA
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
CD274 / B7‑H1 / PD‑L1 Antibody (clone ANC6H1, Azide‑free) LS‑C134638
Species: Human
Applications: Flow Cytometry, ELISA
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
CD274 / B7‑H1 / PD‑L1 Antibody (clone ANC6H1) LS‑C134636
Species: Human
Applications: Flow Cytometry, ELISA
CD274 / B7-H1 / PD-L1 Antibody - Anti-B7-H1 / PD-L1 / CD274 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ CD274 / B7‑H1 / PD‑L1 Antibody (aa261‑290) LS‑B10562
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
CD274 / B7-H1 / PD-L1 Antibody - Surface staining of mouse splenocytes with anti-mouse B7-H1 (MIH5) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
CD274 / B7‑H1 / PD‑L1 Antibody (clone MIH5) LS‑C107430
Species: Mouse
Applications: IHC, IHC - Frozen, Flow Cytometry
See all CD274 / B7-H1 / PD-L1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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