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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7‑H1 / PD‑L1 Antibody (clone MIH6, Low Endotoxin) LS‑C188351
PD-L1 antibody LS-C188351 is an unconjugated rat monoclonal antibody to mouse PD-L1 (CD274 / B7-H1). Validated for Flow, Func, IHC and IP.
Catalog
Size
Price
LS-C188351-500
500 µg (1 mg/ml)
$485
Description
PD-L1 antibody LS-C188351 is an unconjugated rat monoclonal antibody to mouse PD-L1 (CD274 / B7-H1). Validated for Flow, Func, IHC and IP.
Target
Mouse CD274 / B7-H1 / PD-L1
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [MIH6]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Low Endotoxin. Also available Unmodified.
Applications
  • IHC
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry (1:1 - 1:200)
  • Functional Assay
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Mouse CD274 - transfected L5178Y cells.
Specificity
Detects mouse CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
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Images

Flow Cytometry

Flow cytometry of mouse peripheral blood lymphocytes with Rat anti-Mouse CD274:Endotoxin Low This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of mouse peripheral blood lymphocytes with Rat anti-Mouse CD274:Endotoxin Low This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Mouse lymphocytes stained with Rat anti Mouse CD274.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of mouse peripheral blood lymphocytes with Rat anti-Mouse CD274:Endotoxin Low This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of mouse peripheral blood lymphocytes with Rat anti-Mouse CD274:Endotoxin Low This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Mouse lymphocytes stained with Rat anti Mouse CD274.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of mouse peripheral blood lymphocytes with Rat anti-Mouse CD274:Endotoxin Low This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of mouse peripheral blood lymphocytes with Rat anti-Mouse CD274:Endotoxin Low This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Mouse lymphocytes stained with Rat anti Mouse CD274.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of mouse peripheral blood lymphocytes with Rat anti-Mouse CD274:Endotoxin Low This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of mouse peripheral blood lymphocytes with Rat anti-Mouse CD274:Endotoxin Low This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Mouse lymphocytes stained with Rat anti Mouse CD274.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/15/2018
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