PD-L1 antibody LS-C58199 is an unconjugated rat monoclonal antibody to mouse PD-L1 (CD274 / B7-H1). Validated for Flow, IHC and IP.
Mouse CD274 / B7-H1 / PD-L1
(tested or 100% immunogen sequence identity)
Also available conjugated with FITC
Also available Low Endotoxin
- IHC - Frozen
- Flow Cytometry (1:1 - 1:10)
- (applications tested for the base form of this product only)
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Mouse CD274 - transfected L5178Y cells.
Detects mouse CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
PBS, 0.09% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.
About CD274 / B7-H1 / PD-L1