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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7‑H1 / PD‑L1 Antibody (clone MIH6, FITC) LS‑C58198
Note: This antibody replaces LS-C128034
PD-L1 antibody LS-C58198 is an FITC-conjugated rat monoclonal antibody to mouse PD-L1 (CD274 / B7-H1). Validated for Flow, IHC and IP.
Catalog
Size
Price
LS-C58198-100
100 µg (0.1 mg/ml)
$375
Description
PD-L1 antibody LS-C58198 is an FITC-conjugated rat monoclonal antibody to mouse PD-L1 (CD274 / B7-H1). Validated for Flow, IHC and IP.
Target
Mouse CD274 / B7-H1 / PD-L1
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [MIH6]
Conjugations
FITC. Also available Unconjugated.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry (1:1)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Mouse CD274 - transfected L5178Y cells.
Specificity
Detects mouse CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light. Store undiluted.
Restrictions
For research use only.
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

Popular CD274 / B7-H1 / PD-L1 Products

Flow Cytometry Image
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
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Anti-B7-H1 / PD-L1 / CD274 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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Applications: IHC, IHC - Paraffin, Western blot

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Images

Flow Cytometry

Mouse lymphocytes stained with Rat anti Mouse CD274.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Mouse lymphocytes stained with Rat anti Mouse CD274.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Mouse lymphocytes stained with Rat anti Mouse CD274.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Mouse lymphocytes stained with Rat anti Mouse CD274.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Mouse lymphocytes stained with Rat anti Mouse CD274. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Peripheral mouse lymphocytes stained with Rat anti Mouse CD274: FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/18/2018
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