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| Catalog Number | Size | Price |
|---|---|---|
| LS-C669077-0.1 | 0.1 mg (1 mg/ml) | $502 |
![CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [8E12] Blue: DAPI staining](https://lsbio-7d62.kxcdn.com/image2/cd274-b7-h1-pd-l1-antibody-clone-8e12-ls-c669077/484809_3817272.gif)
![CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [8E12] Blue: DAPI staining](https://lsbio-7d62.kxcdn.com/image2/cd274-b7-h1-pd-l1-antibody-clone-8e12-ls-c669077/484808_3817255.gif)
![CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [8E12] Blue: DAPI staining](https://lsbio-7d62.kxcdn.com/image2/cd274-b7-h1-pd-l1-antibody-clone-8e12-ls-c669077/484807_3817238.gif)
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Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone 8E12, IHC, IF, WB) LS‑C669077
Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone 8E12, IHC, IF, WB) LS‑C669077
Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (8E12) Antibody
Application:
IHC-P, ICC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (8E12) Antibody
Application:
IHC-P, ICC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PD-L1 antibody LS-C669077 is an unconjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA, Flow, ICC, IF, IHC and WB.
Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
8E12
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
The extracellular domain of human PD-L1.
Applications
- IHC - Paraffin
- ICC
- Immunofluorescence
- Western blot
- Flow Cytometry
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.25 - 0.5 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 45 kDa
Presentation
PBS containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD274 / B7-H1 / PD-L1
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Species:
Human
Applications:
Flow Cytometry, ELISA
Species:
Human
Applications:
Flow Cytometry, ELISA
Species:
Human
Applications:
Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
IHC, IHC - Frozen, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
