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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7-H1 / PD-L1 Antibody (Biotin) LS-C502432

Biotinylated EB06621 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP block

CD274 / B7-H1 / PD-L1 Antibody (Biotin) LS-C502432

Antibody:
Goat Polyclonal to Human CD274 / B7-H1 / PD-L1
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Biotin, Unmodified
Price
Catalog Number
$405 
LS-C502432-100
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human CD274 / B7-H1 / PD-L1
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Biotin, Unmodified

Specifications

Description
PD-L1 antibody LS-C502432 is a biotin-conjugated goat polyclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for Peptide-ELISA and WB.

Target
Human CD274 / B7-H1 / PD-L1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
Peptide with sequence CKKQSDTHLEET., from the C Terminus of the protein sequence according to NP_054862.1NP_001254635.1.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Requested From: United States
Date Requested: 6/18/2019
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