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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1953-50 | 50 µg (0.5 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human CD177 Antibody (clone MEM‑166, IHC, WB) LS‑B1953
IHC‑plus™ Monoclonal Mouse anti‑Human CD177 Antibody (clone MEM‑166, IHC, WB) LS‑B1953
Note: This antibody replaces LS-C40845
Antibody:
CD177 Mouse anti-Human Monoclonal (MEM-166) Antibody
Application:
IHC, IHC-P, WB, IP, Flo
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CD177 Mouse anti-Human Monoclonal (MEM-166) Antibody
Application:
IHC, IHC-P, WB, IP, Flo
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
CD177 antibody LS-B1953 is an unconjugated mouse monoclonal antibody to CD177 from human. It is reactive with human and monkey. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human CD177
Synonyms
CD177 | CD177 molecule | CD177 antigen | Cell surface receptor | HNA-2a | HNA2A | NB1 | PRV-1 | Polycythemia rubra vera 1 | PRV1 | NB1 glycoprotein | NB1 GP
Host
Mouse
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
MEM-166
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Cells
Specificity
Human granulocytes
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- Immunoprecipitation
- Flow Cytometry (1 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1953 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1953 was determined to be 10 ug/ml.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Storage
Store at 4°C. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD177
Validation
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-CD177 antibody IHC of human intravascular neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD177 antibody IHC of human intravascular neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CD177 Antibody (clone MEM-166) for IHC, WB/Western, IP, Flow LS-B1953 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Expression of neuropeptides and cytokines in a rabbit model of diabetic neuroischemic wound healing. Pradhan Nabzdyk L, Kuchibhotla S, Guthrie P, Chun M, Auster ME, Nabzdyk C, Deso S, Andersen N, Gnardellis C, LoGerfo FW, Veves A. Journal of vascular surgery. 2013 Sep;58:766-75.e12.
CXCL1-LCN2 paracrine axis promotes progression of prostate cancer via the Src activation and epithelial-mesenchymal transition. Lu Y, Dong B, Xu F, Xu Y, Pan J, Song J, Zhang J, Huang Y, Xue W. Cell Communication and Signaling. 2019 September;17:118.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
