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Catalog Number Size Price
LS-C661936-10 10 µg $318 
LS-C661936-100 100 µg $470 
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Western blot analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 / CD135 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Flt3 / CD135 at approximately 160KD. The expected band size for Flt3 / CD135 is at 113KD.
CD135 / FLT3 Antibody - Western blot - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Immunohistochemistry - Anti-Flt3 / CD135 Picoband Antibody
CD135 / FLT3 Antibody - Western blot analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 / CD135 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Flt3 / CD135 at approximately 160KD. The expected band size for Flt3 / CD135 is at 113KD.
CD135 / FLT3 Antibody - Western blot - Anti-Flt3 / CD135 Picoband Antibody
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Polyclonal Rabbit anti‑Mouse CD135 / FLT3 Antibody (IHC, WB) LS‑C661936

Polyclonal Rabbit anti‑Mouse CD135 / FLT3 Antibody (IHC, WB) LS‑C661936

Antibody:
CD135 / FLT3 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661936-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD135 / FLT3 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
FLT3 antibody LS-C661936 is an unconjugated rabbit polyclonal antibody to mouse FLT3 (CD135). Validated for ELISA, IHC and WB.
Target
Mouse CD135 / FLT3
Synonyms
FLT3 | CD135 | CD135 antigen | Fetal liver kinase 2 | Fetal liver kinase-2 | FL cytokine receptor | FLK2 | FLT-3 | Fms-like tyrosine kinase 3 | Stem cell tyrosine kinase 1 | STK-1 | FLK-2 | Fms-related tyrosine kinase 3 | STK1
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse Flt3 / CD135 recombinant protein (Position: E62-E295).
Specificity
Hematopoietic stem and progenitor cell- enriched populations. Found in brain, placenta and testis.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD135 / FLT3
P36888 NM_004119 NP_004110.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024