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Catalog Number Size Price
LS-C59379-1 1 ml (85 mg/ml) $1,225 
CD121b / IL1R2 Antibody - Anti-IL-1RII Antibody - Western Blot. Western blot of anti-IL-1RII antibody. This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown). Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 ( for 45 min at room temperature. Molecular weight markers are shown for size comparison (lane M, red, 700 nm channel). IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Human CD121b / IL1R2 Antibody (WB) LS‑C59379

Polyclonal Rabbit anti‑Human CD121b / IL1R2 Antibody (WB) LS‑C59379

Antibody:
CD121b / IL1R2 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Human, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$1,225
LS-C59379-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD121b / IL1R2 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Human, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
IL1R2 antibody LS-C59379 is an unconjugated rabbit polyclonal antibody to IL1R2 (CD121b) from human. It is reactive with human and primate. Validated for ELISA, IP and WB.
Target
Human CD121b / IL1R2
Synonyms
IL1R2 | CD121b | CD121b antigen | CDw121b | IL-1RT-2 | IL1R2c | Interleukin-1 receptor beta | Interleukin-1 receptor type II | IL-1R-beta | Antigen CDw121b | IL-1 type II receptor | IL-1R-2 | IL-1RT2 | IL1RB | Interleukin-1 receptor type 2
Host
Rabbit
Reactivity
Human, Primate (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type II.
Specificity
This antiserum is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RII. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type I. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type II (also called soluble IL-1R type II) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:400 - 1:800)
  • ELISA (1:1000 - 1:2000)
Usage
This antiserum against Human IL-1 Receptor Type II (IL-1 RII) has been tested for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown. IL-1 RII has been termed p68 or CD121 in some laboratories. IL-1RII has been shown to bind IL-1, IL-1a and IL-1^.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD121b / IL1R2
P27930 NM_004633 NP_004624.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024