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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCNH / Cyclin H Antibody LS‑C669957
Cyclin H antibody LS-C669957 is an unconjugated rabbit polyclonal antibody to human Cyclin H (CCNH). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C669957-50
50 µg
$285
LS-C669957-100
100 µg
$385

Popular CCNH / Cyclin H Products

Immunofluorescence analysis of HeLa cells.
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse
Applications: IHC, ICC, Western blot, Immunoprecipitation, Flow Cytometry
The recombinant proteins (25ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Cyclin H The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: Jurkat cell lysate Lane 3.: Ramos cell lysate Lane 4.: Balb-3T3 cell lysate
Species: Human
Applications: Western blot, ELISA
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Product Description


Specifications

Target
Human CCNH / Cyclin H
Synonyms
CCNH, Cyclin H, p34, p37, CAK, CAK complex subunit, Cyclin-H, MO15-associated protein
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CCNH / Cyclin H antibody was raised against recombinant Human Cyclin-H protein (261-319AA)
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
About CCNH / Cyclin H
CCNH / Cyclin H belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. P51946 NM_001239 NP_001230.1


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Images

Immunohistochemistry - Paraffin

Immunohistochemistry image of paraffin-embedded human adrenal gland tissue at a dilution of 1:100
Immunohistochemistry image of paraffin-embedded human adrenal gland tissue at a dilution of 1:100

Western blot

Positive Western Blot detected in Mouse liver tissue, Rat liver tissue. All lanes: CCNH antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa
Positive Western Blot detected in Mouse liver tissue, Rat liver tissue. All lanes: CCNH antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry image of paraffin-embedded human adrenal gland tissue at a dilution of 1:100
Immunohistochemistry image of paraffin-embedded human adrenal gland tissue at a dilution of 1:100

Western blot

Positive Western Blot detected in Mouse liver tissue, Rat liver tissue. All lanes: CCNH antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa
Positive Western Blot detected in Mouse liver tissue, Rat liver tissue. All lanes: CCNH antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry image of paraffin-embedded human adrenal gland tissue at a dilution of 1:100
Immunohistochemistry image of paraffin-embedded human adrenal gland tissue at a dilution of 1:100

Western blot

Positive Western Blot detected in Mouse liver tissue, Rat liver tissue. All lanes: CCNH antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa
Positive Western Blot detected in Mouse liver tissue, Rat liver tissue. All lanes: CCNH antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry image of paraffin-embedded human adrenal gland tissue at a dilution of 1:100
Immunohistochemistry image of paraffin-embedded human adrenal gland tissue at a dilution of 1:100

Western blot

Positive Western Blot detected in Mouse liver tissue, Rat liver tissue. All lanes: CCNH antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa
Positive Western Blot detected in Mouse liver tissue, Rat liver tissue. All lanes: CCNH antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa

Requested From: United States
Date Requested: 10/16/2018

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